Menopause, a cessation of menstrual bleeding, is directly related to serious illness confined to cardiovascular disease (CVD) such as hypertension and stroke. The oestrogen withdrawal and consumption of high cholesterol diet has a detrimental effect on cardiovascular function and metabolism in menopausal women. The present study was aimed to develop the hypertensive rat model in the ovariectomised Sprague Dawley rats fed with 2% cholesterol diet following a short term period. Eighteen Sprague-Dawley rats were randomly divided into three groups; Sham control (sham), ovariectomized control fed with normal diet (Ovx +N), ovariectomized rats fed with 2% cholesterol diet (Ovx + Cho) for four weeks. At the end of the study, the food intake and body weight were measured. The systolic, diastolic and mean blood pressure was determined. OVX+Cho showed significant increase in physiological changes such as body weight and food intake compared to Ovx+N group. The significant increase in systolic, diastolic and mean blood pressure was observed in Ovx+Cho group. Based on the above findings, it is believed that feeding cholesterol diet for four weeks (short term) results in significant increase in the blood pressure, food intake and body weight in the ovariectomised rats.
Development of Hypertensive Animal Model Using Ovariectomised Rat Fed with Short-Term 2% Cholesterol Diet
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Menopaus merupakan pemberhentian pendarahan haid, ia secara langsung berkaitan dengan penyakit kardiovaskular (CVD) seperti tekanan darah tinggi dan strok. Penghasilan estrogen dan pengambilan diet berkolesterol tinggi mempunyai kesan yang menjejaskan fungsi kardiovaskular dan metabolisma pada wanita. Kajian ini bertujuan untuk menghasilkan model tikus hipertensi dalam tikus Sprague-Dawley terovariektomi yang diberi makan dengan 2% diet kolesterol pada tempoh jangka pendek. Lapan belas tikus Sprague-Dawley dibahagikan secara rawak kepada tiga kumpulan iaitu kumpulan kawalan Sham (sham), kumpulan kawalan terovariektomi diberi diet normal (Ovx + N), tikus terovariektomi diberi diet kolesterol 2% (Ovx + Cho) selama empat minggu. Pada akhir kajian, pengambilan makanan dan berat badan diukur. Tekanan darah sistolik, diastolik dan tekanan min darah ditentukan. OVX + Cho menunjukkan peningkatan yang ketara dalam perubahan fisiologi seperti berat badan dan pengambilan makanan berbanding dengan kumpulan Ovx + N. Peningkatan yang ketara dalam tekanan darah sistolik, diastolik dan tekanan min darah diperhatikan dalam kumpulan Ovx + Cho. Berdasarkan hasil kajian di atas, ia dipercayai bahawa makanan berkolesterol selama empat minggu (jangka pendek) menyebabkan peningkatan ketara dalam tekanan darah, pengambilan makanan dan berat badan dalam tikus terovariektomi.
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